Figure 3

TAC1 expression and DNA methylation in the brain. (A), (B), (C) TAC1 mRNA assayed by quantitative RT-PCR in the striatum (STR), cingulate (CCTX) and temporal cortex (TCTX) of female brains. Data are presented as relative expression of TAC1 compared with ACTB for each sample (circles) and group mean (horizontal bar). *P <0.05; **P <0.01. (D) Scatter plot of DNA methylation at Sequenom (SEQ) CpG site 5 and TAC1 expression in the CCTX stratified by seizure phenotype (RTT/AUT+SEZ) or control (CTL). Regression lines for seizure phenotype (black) and CTL (dashed) indicate an inverse relationship between DNA methylation and expression in CCTX. AUT, autism; RTT, Rett syndrome; SEZ, seizure disorder.