Figure 4

MeCP2 binding to the TAC1 promoter in vivo . (A) Chromatin immunoprecipitation(ChIP) assay in untreated and 3 mM valproic acid (VPA)-treated HEK cells. DNA fragments immunoprecipitated with IgG, anti-MeCP2 or anti-acetyl histone H3 (Ac-H3) were analyzed by PCR with primers specific for the TAC1 promoter locus (Figure 2A). (B) Reduced MeCP2 and increased Ac-H3 occupancy at the TAC1 promoter in DNA extracted from untreated (black) or VPA-treated (grey) HEK cells, assayed in triplicate by quantitative PCR analysis of ChIP samples. Ac-H3 quantification extends beyond the graph to 105% input.