Figure 1

DR6 immunoreactivity (IR) at different gestational ages (13, 17, 23, 25 gestational weeks, GW) and at 1 day postnatally. A-B: 13 GW. A: the hippocampus shows no detectable DR6 IR. B: the cerebral cortex shows weakly reactive fibers in the subplate zone (SP, arrows; high magnification in insert), but no reactivity in the cortical plate (CP), intermediate zone (IZ) or ventricular zone (VZ). C-D: 17 GW. C: light DR6 IR is detected in the hippocampus in the stratum lacunosum-moleculare (SLM). D: cerebral cortex shows no reactivity in the CP. E (23 GW) and F (25 GW) show evident DR6 IR in the different hippocampal subfields (stratum oriens, SO; stratum pyramidale, SP; stratum lacunosum-moleculare, SLM; molecular layer dentate gyrus, ML). G-J: 1 day. G: strong DR6 IR is observed throughout the different hippocampal subfields; high magnification of CA3 and CA1 is shown in H and I, respectively. J: Diffuse and strong DR6 IR is observed throughout the neocortex. Dentate gyrus, DG; granule cell layer, gcl; stratum lucidum, SL. Hematoxylin counterstain shows blue nuclei. Scale bar (shown in J): A, C-G, 400 μm; B, 200 μm; H-I, 40 μm; J, 80 μm.