Figure 1From: Methotrexate treatment of FraX fibroblasts results in FMR1 transcription but not in detectable FMR1 protein levelsFMR1 transcription in fragile X syndrome (FXS) patient and control fibroblasts. cDNA was synthesized from FXS patient fibroblasts and control fibroblasts followed by PCR. A total of 5 μl of PCR products for FMR1 and 3 μl of PCR products for GAPDH were analyzed on a 2% agarose gel.Back to article page