Figure 2
Effect of methotrexate (MTX)-treatment time and dosage on restoration of FMR1 transcription. Patient fibroblasts were treated with MTX and cDNAs were synthesized followed by PCRs. PCRs were analyzed on 2% agarose gels. The upper panels of each figure show analysis of 5 μl FMR1-PCRs, the lower panels show analysis of 3 μl GAPDH-PCRs. a) Cells were treated 4 and 7 days with 0.5, 1, 2 and 4 μg MTX per ml cell culture medium. b) Cells were treated 4, 5, 6 and 8 days with 1 and 2 μg MTX per ml cell culture medium.