Fig. 1

The efficiency of NRXN1 knockdown in vitro and in vivo. A The mRNA level of NRXN1 in primary neurons between Sh-Nc group and Sh-NRXN1 group, P < 0.001, t-test. B The protein level of NRXN1 in primary neurons. C Representative immune-stained images of NRXN1 in rat neurons. Scale bar, 50μm. D The image of GFP-reporter virus vectors in the prelimbic region of the PFC in rats. E The protein expression of NRXN1 was detected by immunohistochemistry (IHC) in brain sections of rats; representative images of the virus-affected region and unaffected region are displayed, respectively. F The protein level of NRXN1 in SD rats. F (2, 6) = 51.59, P < 0.001, one-way ANOVA. These data were presented as mean ± SD of three independent experiments. *P < 0.05, **P < 0.01, ***P < 0.001 versus WT group. #P < 0.05, ##P < 0.01, ###P < 0.001 versus Sh-Nc group