Fig. 4
From: Role for TGF-β superfamily signaling in telencephalic GABAergic neuron development
Role for TGF-β signaling in tangential migration of GABAergic interneurons in the developing telencephalon. Organotypic cultures of E12.5 forebrain slices from different levels of the rostrocaudal axis were focally electroporated into the MGE or CGE with a GFP expression vector alone (left hemisphere; panels a-l) or with a combination of GFP and dominant-negative Smad4 (Smad4-DN) (right hemisphere; shown in each case in panels a′-l′). Slices were photographed after 12, 36, 60 and 84 h to visualize the tangential migration of GFP-expressing (i.e., electroporated) cells away from the MGE or CGE. A significant decrease of cell migration towards the neocortex was observed in the hemisphere electroporated with Smad4-DN