Fig. 4

The transcription factor GTF2IRD1 is expressed in layer V neurons of the prefrontal cortex. Top, the blue rectangle on the schematic depicts the region of prefrontal cortex shown in the images below. Coronal sections of prefrontal cortex from an adult Gtf2ird1^(XS0608)Wtsi mouse (left) and a control Gtf2ird1^+/+ mouse (right) stained for LacZ activity. WM denotes white matter, and cortical layers are indicated in roman numerals. Scale bar: 300 µm. Higher magnification images illustrate the laminar pattern of staining in medial prefrontal cortex from the regions shown above in black rectangles. Note the somatic expression of the GTF2IRD1-LacZ fusion protein in layer V neurons (examples shown with arrowheads). Scale bar: 50 µm