Fig. 2
From: TPH2 polymorphisms and expression in Prader-Willi syndrome subjects with differing genetic subtypes
Quantitative RT-PCR analysis of TPH2 mRNA level in PWS 15q11-q13 deletion subjects (n = 6), PWS maternal disomy (UPD) subjects (n = 6), and control subjects (n = 12). PWS deletion subjects all showed lower TPH2 expression compared with PWS UPD or control subjects. RQ is the normalized expression value (RQ = 2–[ ΔΔC ]T ), and higher RQ values represent increased gene expression. TPH2 expression values were normalized to GAPDH (see Materials and Methods section for details). Data are stratified by the genotype at three separate SNP loci: -703G/T; -473T/A; +90A/G (wild type alleles are in bold-face type)