Figure 3
Quantitative PCR confirmation of genes identified as altered by neurodevelopmental ethanol exposure by microarray analysis. Expression values are shown as a ratio of the control value ± SEM. Microarray values represent the results of ANOVA comparison of two biological replicates, each consisting of the RNA from three non-littermate male mice. Quantitative PCR values were generated using six biological replicates consisting of the RNA from six ethanol-exposed males mice. Significance differences from control values were determined using unpaired t-tests. All bars are significantly different from their respective controls (P <0.05). We were unable to confirm the expression of Htr5a (P >0.05) and therefore this gene is not included in this figure.