Figure 2

Reduction in brain size, cortical thinning and smaller ventricles in the irradiated embryonic brain. (A, C, F) Representative images are shown for the neocortex at 24 h after irradiation (A) and for the cortex (C) and the whole brain (F) at 1 week post irradiation with 1.0 Gy, with the indication of the lateral ventricle (LV) and the total brain area (outlined in (F)). (B, D, E, G) Morphometric measurements showed a reduction in neocortical thickness at 24 h (B) after radiation exposure. Furthermore, at 1 week post irradiation with 1.0 Gy of X-rays, the thickness of the cortex (D), as well as the total brain volume (E) and the ventricle area (G), was significantly reduced in the irradiated embryos. Data are presented as mean ± SEM. **P < 0.01, ***P < 0.001 for comparison with controls. The number of animals used per test is indicated in the graphs (N). Scale bar: 100 μm in (A) and (C), 1,000 μm in (F).