Figure 4

Prenatal radiation exposure of 1.0 Gy at E11 impairs juvenile hippocampal neurogenesis. (A) An overview of hippocampal neurogenic stages is shown, with the indication of immunohistochemical markers used in our study: Sox2, Ki67, PCNA, GFAP and DCX. (B) Quantification of these endogenous markers was performed using either the subgranular zone (SGZ) area (for GFAP) or the length of the dentate gyrus (DG) (for the other markers). (C-L) We showed a decrease in Sox2-positive (C, D) and in Ki67-positive (E, F) cells, but no change in PCNA (G, H), GFAP (I, J) and DCX (K, L) expression when compared to controls. Data are presented as mean ± SEM. *P < 0.05 for comparison with controls. The number of animals used per test is indicated in the graphs (N). Scale bar: 10 μm.