Table 1 Behavioral characterization of WT and Met^fx/fx/Emx1^cre KO mice

Activity chamber

Genotype, F (1, 51) = 12.26

p < 0.05

Total distance traveled(cm)

3317.67 ± 218.98

2339.74 ± 175.11

Time, F (5, 255) = 4.308

p < 0.001

Interaction, F (5, 255) = 3.184

p < 0.01

Steady speed rotarod

Genotype, F (1, 52) = 4.249

p < 0.05

Average latency to fall(sec)

20.0185 ± 2.58

28.481 ± 3.19

Trial, F (9, 468) = 18.99

p < 0.0001

Interaction, F (9, 468) = 1.063

p > 0.05

Accelerating rotarod

Genotype, F (1, 12) = 0.017

p > 0.05

Average latency to fall(sec)

151.43 ± 19.82

148.10 ± 16.14

Trial, F (2, 24) = 15.35

p < 0.0001

Interaction, F (2, 24) = 0.024

p > 0.05

T-maze

% SA

54.701 ± 1.679

47.4956 ± 2.401

t ₅₁ = 2.475

p < 0.05

% AAR

35.063 ± 1.554

38.576 ± 1.599

t ₅₁ = −1.576

p > 0.05

% SAR

3.891 ± 0.666

5.301 ± 0.907

t ₅₁ = −1.410

p > 0.05

#arm entries

35.852 ± 2.456

29.154 ± 1.859

t ₅₁ = 2.162

p < 0.05

Marble burying (#)

7.100 ± 1.567

9.200 ± 1.756

t ₁₈ = 0.8923

p > 0.05

EPM entries (#)

Genotype, F (1, 32) = 0.2539

p > 0.05

Closed arms

9.188 ± 1.697

8.882 ± 1.364

Arm entry, F (1, 32) = 65.93

p < 0.05

Open arms

1.706 ± 0.803

0.5882 ± 0.298

Interaction, F (1, 32) = 0.2081

p > 0.05

EPM arm duration (%)

Genotype, F (1, 32) = 0.3463

p > 0.05

Closed arms

88.882 ± 3.169

86.863 ± 5.017

Arm duration, F (1, 32) = 220.8

p < 0.0001

Open arms

4.3137 ± 1.505

3.000 ± 1.649

Interaction, F (1, 32) = 0.2508

p > 0.05

Sociability (sec)

Genotype, F (1, 52) = 1.463

p > 0.05

Side with mouse

312.897 ± 14.864

317.225 ± 11.721

Chamber side, F (1, 52) = 74.25

p < 0.05

Side with empty cup

185.271 ± 12.428

162.44 ± 11.885

Interaction, F (1, 52) = 0.6864

p > 0.05

Social novelty (sec)

Genotype, F (1, 52) = 0.001

p > 0.05

Familiar mouse side

227.609 ± 13.819

235.362 ± 17.602

Chamber side, F (1, 52) = 1.545

p > 0.05

Novel mouse side

259.723 ± 14.579

252.667 ± 14.533

Interaction, F (1, 52) = 0.1387

p > 0.05

Sociability (sec)

Genotype, F (1, 52) = 0.005652

p > 0.05

Cup with mouse

62.795 ± 4.653

60.152 ± 5.095

Cup, F (1, 52) = 214.6

p < 0.05

Empty cup

11.818 ± 1.606

12.645 ± 1.254

Interaction, F (1, 52) = 0.2665

p > 0.05

Social novelty (sec)

Genotype, F (1, 52) = 0.02144

p > 0.05

Cup with familiar mouse

23.087 ± 3.234

21.624 ± 2.801

Cup, F (1, 52) = 63.83

p < 0.05

Cup with novel mouse

46.756 ± 5.329

46.817 ± 4.221

Interaction, F (1, 52) = 0.06198

p > 0.05

Contextual fear (%)

Baseline

0.04976 ± 0.0497

0.3007 ± 0.1955

Genotype, F (1, 31) = 0.6584

p > 0.05

Training

28.73 ± 3.2649

32.72 ± 2.6444

Trial, F (2, 62) = 110.5

p < 0.0001

Test

29.04 ± 3.2954

30.81 ± 3.4346

Interaction, F (2, 62) = 0.3224

p > 0.05