Skip to main content

Table 2 Behavioral characterization of WT, Metfx/+/Nestincre Het, and Metfx/fx/Nestincre KO mice

From: Complete or partial reduction of the Met receptor tyrosine kinase in distinct circuits differentially impacts mouse behavior

  WT Metfx/+/Nestincre Het Metfx/fx/Nestincre KO F Statistic p value
Activity chamber      
     Genotype, F (2, 55) = 2.491 p > 0.05
Total distance traveled(cm) 2807.3 ± 197.9 3445.5 ± 236.0 2842.9 ± 140.5 Time, F (5, 275) = 18.09 p < 0.0001
     Interaction, F (5, 275) = 1.410 p > 0.05
Steady speed rotarod      
     Genotype, F (2, 55) = 1.971 p > 0.05
Latency to fall(sec) 27.417 ± 2.635 14.271 ± 2.016 20.033 ± 3.363 Trial, F (9, 495) = 9.686 p < 0.0001
     Interaction, F (9, 495) = 1.002 p > 0.05
Accelerating rotarod      
     Genotype, F (2, 17) = 0.033 p > 0.05
Latency to fall(sec) 150.39 ± 15.80 155.49 ± 14.76 148.38 ± 14.71 Trial, F (2, 34) = 18.50 p < 0.0001
     Interaction, F (2, 34) = 1.737 p > 0.05
T-maze      
% SA 44.837 ± 2.456 49.408 ± 2.568 50.113 ± 2.181 F (2, 53) = 1.417 p > 0.05
% AAR 36.794 ± 2.114 31.852 ± 1.356 31.123 ± 2.096 F (2, 53) = 2.408 p > 0.05
% SAR 7.354 ± 1.1011 7.840 ± 1.198 8.192 ± 1.170 F (2, 53) = 0.1350 p > 0.05
#arm entries 27.963 ± 1.778 30.429 ± 2.467 33.333 ± 2.131 F (2, 53) = 1.742 p > 0.05
Marble burying (#) 8 ± 1.076 10 ± 1.742 7.0 ± 1.366 F (2, 53) = 0.6750 p > 0.05
EPM entries (#)      
     Genotype, F (2, 54) = 1.490 p > 0.05
Closed arms 9.5 ± 0.946 11.21 ± 1.085 9.8 ± 1.147 Arm entries, F (1, 54) = 147.8 p < 0.0001
Open arms 1.75 ± 0.413 3.21 ± 0.594 2.4 ± 0.523 Interaction, F (2, 54) = 0.06371 p > 0.05
EPM duration (%)      
     Genotype, F (2, 54) = 0.0 p > 0.05
Closed arms 81.037 ± 2.725 63.268 ± 5.701 75.057 ± 3.494 Arm duration, F (2, 108) = 228.1 p < 0.0001
Open arms 8.102 ± 2.276 13.523 ± 5.628 8.736 ± 2.077 Interaction, F (2, 108) = 4.299 p < 0.005
Sociability (sec)      
     Genotype, F (2, 53) = 1.032 p > 0.05
Side with mouse 335.257 ± 12.743 340.71 ± 11.666 310.944 ± 13.768 Chamber side, F (2, 106) = 197.6 p < 0.0001
Side with empty cup 165.806 ± 10.710 144.05 ± 10.953 199.303 ± 14.887 Interaction, F (2, 106) = 2.493 p < 0.05
Social novelty (sec)      
     Genotype, F (2, 53) = 0.4669 p > 0.05
Familiar mouse side 239.276 ± 15.473 275.184 ± 20.999 239.899 ± 24.126 Chamber side, F (2, 106) = 47.53 p < 0.0001
Novel mouse side 256.915 ± 14.165 213.617 ± 17.351 256.977 ± 24.069 Interaction, F (2, 106) = 1.174 p > 0.05
Sociability (sec)      
     Genotype, F (2, 53) = 0.7821 p > 0.05
Cup with mouse 76.390 ± 5.722 72.092 ± 5.533 64.901 ± 5.795 Cup time, F (1, 53) = 212.2 p < 0.0001
Empty cup 13.486 ± 1.589 12.605 ± 2.816 14.391 ± 2.338 Interaction, F (2, 53) = 0.9274 p > 0.05
Social novelty (sec)      
     Genotype, F (2, 53) = 0.2960 p > 0.05
Cup with familiar mouse 25.686 ± 3.224 27.324 ± 3.362 25.807 ± 3.938 Cup time, F (1, 53) = 23.81 p < 0.0001
Cup with novel Mouse 46.11 ± 3.858 38.358 ± 4.95 41.836 ± 4.558 Interaction, F (2, 53) = 0.7666 p > 0.05
Cue fear training (%)      
     Genotype, F (2, 21) = 1.206 p > 0.05
Average freezing 27.681 ± 3.621 20.959 ± 9.090 24.956 ± 2.421 Trial, F (4, 84) = 31.76 p < 0.0001
     Interaction, F (4, 84) = 0.8341 p > 0.05
Cue fear context test (%)      
Average freezing 25.501 ± 2.778 21.132 ± 6.485 24.408 ± 4.978 F (2, 21) = 0.201 p > 0.05
Cue fear cue test (%)      
     Genotype, F (2, 21) = 3.156 p > 0.05
Average freezing 61.120 ± 1.062 72.072 ± 2.478 78.351 ± 2.758 Trial, F (9, 189) = 1.398 p > 0.05
     Interaction, F (9, 189) = 0.9551 p > 0.05
Cue fear extinction 1 (%)      
     Genotype, F (2, 21) = 1.032 p > 0.05
Average freezing 47.999 ± 1.515 60.181 ± 2.219 56.860 ± 2.118 Trial, F (9, 189) = 1.975 p < 0.05
     Interaction, F (9, 189) = 0.6945 p > 0.05
Extinction 2      
     Genotype, F (2, 21) = 0.9524 p > 0.05
Average freezing 34.572 ± 1.967 40.725 ± 3.196 45.103 ± 1.575 Trial, F (9, 189) = 1.480 p > 0.05
     Interaction, F (9, 189) = 1.196 p > 0.05
Extinction 3      
     Genotype, F (2, 21) = 0.2070 p > 0.05
Average freezing 30.900 ± 2.513 29.381 ± 1.754 36.167 ± 1.686 Trial, F (9, 189) = 1.026 p > 0.05
     Interaction, F (9, 189) = 1.180 p > 0.05