Table 2 Behavioral characterization of WT, Met^fx/+/Nestin^cre Het, and Met^fx/fx/Nestin^cre KO mice

Activity chamber

Genotype, F (2, 55) = 2.491

p > 0.05

Total distance traveled(cm)

2807.3 ± 197.9

3445.5 ± 236.0

2842.9 ± 140.5

Time, F (5, 275) = 18.09

p < 0.0001

Interaction, F (5, 275) = 1.410

p > 0.05

Steady speed rotarod

Genotype, F (2, 55) = 1.971

p > 0.05

Latency to fall(sec)

27.417 ± 2.635

14.271 ± 2.016

20.033 ± 3.363

Trial, F (9, 495) = 9.686

p < 0.0001

Interaction, F (9, 495) = 1.002

p > 0.05

Accelerating rotarod

Genotype, F (2, 17) = 0.033

p > 0.05

Latency to fall(sec)

150.39 ± 15.80

155.49 ± 14.76

148.38 ± 14.71

Trial, F (2, 34) = 18.50

p < 0.0001

Interaction, F (2, 34) = 1.737

p > 0.05

T-maze

% SA

44.837 ± 2.456

49.408 ± 2.568

50.113 ± 2.181

F (2, 53) = 1.417

p > 0.05

% AAR

36.794 ± 2.114

31.852 ± 1.356

31.123 ± 2.096

F (2, 53) = 2.408

p > 0.05

% SAR

7.354 ± 1.1011

7.840 ± 1.198

8.192 ± 1.170

F (2, 53) = 0.1350

p > 0.05

#arm entries

27.963 ± 1.778

30.429 ± 2.467

33.333 ± 2.131

F (2, 53) = 1.742

p > 0.05

Marble burying (#)

8 ± 1.076

10 ± 1.742

7.0 ± 1.366

F (2, 53) = 0.6750

p > 0.05

EPM entries (#)

Genotype, F (2, 54) = 1.490

p > 0.05

Closed arms

9.5 ± 0.946

11.21 ± 1.085

9.8 ± 1.147

Arm entries, F (1, 54) = 147.8

p < 0.0001

Open arms

1.75 ± 0.413

3.21 ± 0.594

2.4 ± 0.523

Interaction, F (2, 54) = 0.06371

p > 0.05

EPM duration (%)

Genotype, F (2, 54) = 0.0

p > 0.05

Closed arms

81.037 ± 2.725

63.268 ± 5.701

75.057 ± 3.494

Arm duration, F (2, 108) = 228.1

p < 0.0001

Open arms

8.102 ± 2.276

13.523 ± 5.628

8.736 ± 2.077

Interaction, F (2, 108) = 4.299

p < 0.005

Sociability (sec)

Genotype, F (2, 53) = 1.032

p > 0.05

Side with mouse

335.257 ± 12.743

340.71 ± 11.666

310.944 ± 13.768

Chamber side, F (2, 106) = 197.6

p < 0.0001

Side with empty cup

165.806 ± 10.710

144.05 ± 10.953

199.303 ± 14.887

Interaction, F (2, 106) = 2.493

p < 0.05

Social novelty (sec)

Genotype, F (2, 53) = 0.4669

p > 0.05

Familiar mouse side

239.276 ± 15.473

275.184 ± 20.999

239.899 ± 24.126

Chamber side, F (2, 106) = 47.53

p < 0.0001

Novel mouse side

256.915 ± 14.165

213.617 ± 17.351

256.977 ± 24.069

Interaction, F (2, 106) = 1.174

p > 0.05

Sociability (sec)

Genotype, F (2, 53) = 0.7821

p > 0.05

Cup with mouse

76.390 ± 5.722

72.092 ± 5.533

64.901 ± 5.795

Cup time, F (1, 53) = 212.2

p < 0.0001

Empty cup

13.486 ± 1.589

12.605 ± 2.816

14.391 ± 2.338

Interaction, F (2, 53) = 0.9274

p > 0.05

Social novelty (sec)

Genotype, F (2, 53) = 0.2960

p > 0.05

Cup with familiar mouse

25.686 ± 3.224

27.324 ± 3.362

25.807 ± 3.938

Cup time, F (1, 53) = 23.81

p < 0.0001

Cup with novel Mouse

46.11 ± 3.858

38.358 ± 4.95

41.836 ± 4.558

Interaction, F (2, 53) = 0.7666

p > 0.05

Cue fear training (%)

Genotype, F (2, 21) = 1.206

p > 0.05

Average freezing

27.681 ± 3.621

20.959 ± 9.090

24.956 ± 2.421

Trial, F (4, 84) = 31.76

p < 0.0001

Interaction, F (4, 84) = 0.8341

p > 0.05

Cue fear context test (%)

Average freezing

25.501 ± 2.778

21.132 ± 6.485

24.408 ± 4.978

F (2, 21) = 0.201

p > 0.05

Cue fear cue test (%)

Genotype, F (2, 21) = 3.156

p > 0.05

Average freezing

61.120 ± 1.062

72.072 ± 2.478

78.351 ± 2.758

Trial, F (9, 189) = 1.398

p > 0.05

Interaction, F (9, 189) = 0.9551

p > 0.05

Cue fear extinction 1 (%)

Genotype, F (2, 21) = 1.032

p > 0.05

Average freezing

47.999 ± 1.515

60.181 ± 2.219

56.860 ± 2.118

Trial, F (9, 189) = 1.975

p < 0.05

Interaction, F (9, 189) = 0.6945

p > 0.05

Extinction 2

Genotype, F (2, 21) = 0.9524

p > 0.05

Average freezing

34.572 ± 1.967

40.725 ± 3.196

45.103 ± 1.575

Trial, F (9, 189) = 1.480

p > 0.05

Interaction, F (9, 189) = 1.196

p > 0.05

Extinction 3

Genotype, F (2, 21) = 0.2070

p > 0.05

Average freezing

30.900 ± 2.513

29.381 ± 1.754

36.167 ± 1.686

Trial, F (9, 189) = 1.026

p > 0.05

Interaction, F (9, 189) = 1.180

p > 0.05