Fig. 4
From: Genomic sequencing of a dyslexia susceptibility haplotype encompassing ROBO1
EMSA for SNV4. Nuclear extracts from HEK293 cells overexpressing LHX2-V5 or GFP-V5 were used. The arrow shows the location of the bands that showed differences in the amount of probe bound in a protein-DNA complex. A mixture of two antibodies against LHX2 was used to confirm that the protein-DNA complex at the level indicated by the arrow contains LHX2. An unlabeled probe was used to compete the binding of LHX2 to the SNV probes. The T allele seemed to be more resistant than the C allele to the competing probe as seen when comparing the bands above the asterisks