Fig. 1

Western blot and immunofluorescence results for HEK cells. a Western blot image showing the overexpression of tagged NFXL1 in cells transfected with the NFXL1 plasmid compared to control cells. Samples of two micrograms of protein lysate were loaded onto the gel. Exposure time was 20 s. The protein ladder is superimposed with the WB image. The endogenous NFXL1 is expected to have a mass of 101 kDa. b Immunofluorescence of successfully transfected HEK cells showing overexpressed NFXL1 with the antibody for the endogenous protein. c Immunofluorescence of successfully transfected HEK cells showing overexpressed NFXL1 with the antibody for the DDK tag. d Superimposed images showing signals from both antibodies and a DAPI staining of the cell nuclei