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Table 2 Summary of genotype differences in the hippocampus

From: Effects of sex and DTNBP1 (dysbindin) null gene mutation on the developmental GluN2B-GluN2A switch in the mouse cortex and hippocampus

Protein measure

Brain region

Number

Number of animals of each DTNBP1 genotype per age groupa (P7, 14, 28, 56)

Genotype differences

DTNBP1 −/− (null) [mean]

DTNBP1 +/+ (WT) [mean]

ANOVA F statistic (main effects of genotype, age and sex; genotype × age interaction, genotype × sex interaction

ANOVA p value

PSD enrichments

 

GluN2B:GluN1

HIP

80

10 (−/−)10 (−/−)

Genotype × age interaction

P7; 1.04

P7; 1.17

Genotype F(1, 64) = 1.9

N.S.

10 (+/+)

P14; 1.11

P14; 0.95

Age F(3, 64) = 7.2

p < 0.0005

P28; 0.99

P28; 0.89

Sex F(1, 64) = 0.3

N.S.

P56; 0.94

P56; 0.88

G × A interaction F(3, 64) = 3.9

p < 0.05

G × S interaction F(1, 64) = 0.1

N.S.

GluN2A:GluN1

HIP

80

10 (−/−)

DTNBP1(−/−) > WT

1.06

0.94

Genotype F(1, 64) = 5.3

p < 0.05

10 (+/+)

Age F(3, 64) = 7.9

p < 0.0005

Sex F(1, 64) = 1.7

N.S.

G × A interaction F(3, 64) = 0.6

N.S.

G × S interaction F(1, 64) = 0.1

N.S.

Fyn

HIP

80b

10 (−/−)

WT > DTNBP1(−/−) females only

F; 0.77

F; 1.24

Genotype F(1, 64) = 11.1

p < 0.005

10 (+/+)

M; 0.98

M; 1.03

Age F(3, 64) = 25.0

p < 0.00001

Sex F(1, 64) = 0.1

N.S.

G × A interaction F(3, 64) = 2.1

N.S.

G × S interaction F(1, 64) = 6.0

p < 0.05

pY527-Src

HIP

80

10 (−/−)

WT > DTNBP1(−/−)

0.92

1.08

Genotype F(1, 64) = 5.7

p < 0.05

10 (+/+)

Age F(3, 64) = 21.0

p < 0.00001

Sex F(1, 64) = 0.1

N.S.

G × A interaction F(3, 64) = 1.0

N.S.

G × S interaction F(1, 64) = 3.6

(p = 0.06)

PLCγ

HIP

80

10 (−/−)

WT > DTNBP1(−/−)

0.97

1.02

Genotype F(1, 64) = 5.1

p < 0.05

10 (+/+)

Age F(3, 64) = 2.5

N.S.

Sex F(1, 64) = 1.7

N.S.

G × A interaction F(3, 64) = 2.3

N.S.

G × S interaction F(1, 64) = 0.1

N.S.

  1. N number of samples, P postnatal days, PSD postsynaptic density enrichments, HIP hippocampus, WT wild-type, G genotype, A age, S sex, N.S. not significant
  2. aIncludes both males and females in each age/genotype group
  3. bData log transformed prior to analysis