Protein measure | Brain region | Number | Number of animals of each DTNBP1 genotype per age groupa (P7, 14, 28, 56) | Genotype differences | DTNBP1 −/− (null) [mean] | DTNBP1 +/+ (WT) [mean] | ANOVA F statistic (main effects of genotype, age and sex; genotype × age interaction, genotype × sex interaction | ANOVA p value |
---|---|---|---|---|---|---|---|---|
PSD enrichments | ||||||||
GluN2B:GluN1 | HIP | 80 | 10 (−/−)10 (−/−) | Genotype × age interaction | P7; 1.04 | P7; 1.17 | Genotype F(1, 64) = 1.9 | N.S. |
10 (+/+) | P14; 1.11 | P14; 0.95 | Age F(3, 64) = 7.2 | p < 0.0005 | ||||
P28; 0.99 | P28; 0.89 | Sex F(1, 64) = 0.3 | N.S. | |||||
P56; 0.94 | P56; 0.88 | G × A interaction F(3, 64) = 3.9 | p < 0.05 | |||||
G × S interaction F(1, 64) = 0.1 | N.S. | |||||||
GluN2A:GluN1 | HIP | 80 | 10 (−/−) | DTNBP1(−/−) > WT | 1.06 | 0.94 | Genotype F(1, 64) = 5.3 | p < 0.05 |
10 (+/+) | Age F(3, 64) = 7.9 | p < 0.0005 | ||||||
Sex F(1, 64) = 1.7 | N.S. | |||||||
G × A interaction F(3, 64) = 0.6 | N.S. | |||||||
G × S interaction F(1, 64) = 0.1 | N.S. | |||||||
Fyn | HIP | 80b | 10 (−/−) | WT > DTNBP1(−/−) females only | F; 0.77 | F; 1.24 | Genotype F(1, 64) = 11.1 | p < 0.005 |
10 (+/+) | M; 0.98 | M; 1.03 | Age F(3, 64) = 25.0 | p < 0.00001 | ||||
Sex F(1, 64) = 0.1 | N.S. | |||||||
G × A interaction F(3, 64) = 2.1 | N.S. | |||||||
G × S interaction F(1, 64) = 6.0 | p < 0.05 | |||||||
pY527-Src | HIP | 80 | 10 (−/−) | WT > DTNBP1(−/−) | 0.92 | 1.08 | Genotype F(1, 64) = 5.7 | p < 0.05 |
10 (+/+) | Age F(3, 64) = 21.0 | p < 0.00001 | ||||||
Sex F(1, 64) = 0.1 | N.S. | |||||||
G × A interaction F(3, 64) = 1.0 | N.S. | |||||||
G × S interaction F(1, 64) = 3.6 | (p = 0.06) | |||||||
PLCγ | HIP | 80 | 10 (−/−) | WT > DTNBP1(−/−) | 0.97 | 1.02 | Genotype F(1, 64) = 5.1 | p < 0.05 |
10 (+/+) | Age F(3, 64) = 2.5 | N.S. | ||||||
Sex F(1, 64) = 1.7 | N.S. | |||||||
G × A interaction F(3, 64) = 2.3 | N.S. | |||||||
G × S interaction F(1, 64) = 0.1 | N.S. |