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Fig. 3 | Journal of Neurodevelopmental Disorders

Fig. 3

From: Functional characterization of rare FOXP2 variants in neurodevelopmental disorder

Fig. 3

Interaction of rare FOXP2 variants with CTBP1/2. a, b BRET assays for interaction of FOXP proteins with CTBP1 and CTBP2. HEK293 cells were transfected with FOXP1, FOXP2, or FOXP4 fused to Renilla luciferase (donor) and CTBP1 (a) or CTBP2 (b) fused to YFP (acceptor). The control donor protein is a nuclear-targeted luciferase. Values are mean corrected BRET ratios ± S.D. (n = 3). Asterisks indicate significant differences compared to control (p < 0.05, one-way ANOVA followed by Bonferroni post hoc correction). NS not significant. Exact p values for both a and b are <0.0001 for FOXP2 and FOXP1 and >0.9999 for FOXP4. c Fluorescence micrographs of HEK293 cells transfected with mCherry-CTBP1 or mCherry-CTBP2, together with YFP-FOXP2. Nuclei were stained with Hoechst 33342. d, e BRET assays for interaction of rare FOXP2 variants with CTBP1 and CTBP2. HEK293 cells were transfected with FOXP2 variants fused to Renilla luciferase (donor) and CTBP1 (d) or CTBP2 (e) fused to YFP (acceptor). The control donor protein is a nuclear-targeted luciferase. Values are mean corrected BRET ratios ± S.D. (n = 3). Asterisks indicate significant differences compared to wild-type (WT) FOXP2 (p < 0.05, one-way ANOVA followed by Bonferroni post hoc correction). NS not significant. Exact p values for d are <0.0001 for the control and the R553H, R328* and Q390Vfs*7 variants, 0.221 for the M406T variant, and >0.9999 for all other variants. Exact p values for e are <0.0001 for the control and the R553H, R328* and Q390Vfs*7 variants and >0.9999 for all other variants. f, g Fluorescence micrographs of HEK293 cells transfected with YFP-FOXP2 and mCherry-CTBP1 (f) or mCherry-CTBP2 (g). Nuclei were stained with Hoechst 33342

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