Fig. 1
Experimental setup and baseline data. a Schematic of intrinsic signal optical imaging (ISOI) setup. Visual stimuli were presented to the left eye of a lightly anesthetized mouse via an LCD screen. The craniotomy over right visual cortex was illuminated with 700-nm light and changes in reflectance were captured with a CCD camera. b The tandem lens macroscope was focused 600 μm into the brain from the surface vasculature (left, shown before focusing into the brain). Magnitude and phase maps (right) were extracted using Fourier analysis of each pixel’s time course. Scale bars are 1 mm. c Example retinotopic maps from a male WT P101 mouse depicting six HVAs. d Although male mice were systematically larger, body weights were similar within sex and across genotypes (two-way ANOVA, effect of genotype F (2,36) = 0.519, p = 0.6; effect of sex F (1,36) = 54.5, p < 0.0001). e No significant differences in age were detected (two-way ANOVA, effect of genotype F (2,36) = 0.772, p = 0.47; effect of sex F (1,36) = 0.492, p = 0.49)