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Fig. 7 | Journal of Neurodevelopmental Disorders

Fig. 7

From: Acamprosate in a mouse model of fragile X syndrome: modulation of spontaneous cortical activity, ERK1/2 activation, locomotor behavior, and anxiety

Fig. 7

Locomotor activity and acoustic startle habituation/prepulse inhibition. Wild-type and Fmr1 KO littermates were treated chronically with either saline or 122.2 mg/kg CaCl2 (_Controls; equivalent amount of Ca2+ ions as in the 300 mg/kg acamprosate treated group) or 300 mg/kg acamprosate (+Acamp). For locomotor activity, a three-way ANOVA with a repeated factor of interval (auto regressive (AR) (1)) revealed main effects of interval and a gene×drug interaction for beam breaks during a 60-min open field test. Panel a shows number of beam breaks at each 5-min interval, however, since there was no interaction of interval, pairwise comparisons were performed on beam break data collapsed across time (b). Pairwise comparisons corrected using FDR (two-tailed) demonstrated KO_Controls accumulated more beam breaks than WT_Controls, indicating a baseline increase in locomotor behavior in the KO mice. The KO + Acamp mice had reduced beam breaks compared to KO_Controls, indicating a significant effect of acamprosate treatment in the KO mice. No differences between control treatment and acamprosate treatment were evident in the WT mice. In the startle habituation paradigm, a three-way repeated measures ANOVA (AR (1)) for Vmax revealed a main effect of drug. Pairwise comparisons did not reveal any significant group differences that were maintained following FDR correction (two-tailed) (c). For % inhibition during PPI trials, a three-way mixed factor ANOVA with gene and drug as between factors and trial type (PPI73, PPI77, PPI82: PPIxx) as a within factor was used but the omnibus ANOVA did not reveal any significant effects (d). For locomotor: WT_Controls (n = 24), WT + Acamp (n = 11), KO_Controls (n = 20), KO + Acamp (n = 11). For Habituation: WT_Controls (n = 22), WT + Acamp (n = 11), KO_Controls (n = 20), KO + Acamp (n = 11). For % PPI: WT_Controls (n = 23), WT + Acamp (n = 11), KO_Controls (n = 20), KO + Acamp (n = 11). Data shown are LS mean ± SEM; *p < 0.05, †p < 0.1; N.S. = not significant

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