Fig. 4

Postnatal motor neuron and interneuron analyses. Representative confocal images of the ventral horn of lumbar sections in the P60 spinal cord from both euploid and Ts65n mice, stained for a acetyl choline transferase (ChAT), b calbindin (CB), c parvalbumin (PV), and d calretinin (CR). e At P60, qRT-PCR gene expression analysis shows a significant increase in expression level of Hb9 and a significant decrease in the expression of Irx3 in the trisomic spinal cords (n = 3 euploid and 3 Ts65Dn; *p < 0.05). f At P60, there is a significant increase in the number of CR+ cells in the trisomic animals (n = 4 euploid and 4 Ts65Dn; *p < 0.05) while at g 10–11 months of age, there are significantly fewer ChAT+ motor neurons and CB+ interneurons in the trisomic animals (n = 4 euploid and 6 Ts65Dn, *p < 0.05)