Table 1 Primer sequences for genotyping of embryonic and adult mice and sex-determination of embryonic mice
| Â | Target sequence | Internal control sequence |
|---|---|---|
Ts65Dn (PCR) | Chr17fwd: GTGGCAAGAGACTCAAATTCAAC | IMR8545: AAAGTCGCTCTGAGTTGTTAT |
Chr16rev: TGGCTTATTATTATCAGGGCATTT | IMR8546: GGAGCGGGAGAAATGGATATG | |
Ts65Dn (qPCR) | Appfwd: TGCTGAAGATGTGGGTTCGA | Apobfwd: CACGTGGGCTCCAGCATT |
Apprev: GACAATCACGGTTGCTATGACAA | Apobrev: TCACCAGTCATTTCTGCCTTTG | |
AppProbe: FAM- CAAAGGCGCCATCATCGGACTCA-TAMRA | ApobProbe: VIC- CCAATGGTCGGGCACTGCTCAA-TAMRA | |
Sex determination | SRY-fwd: GCTGGGATGCAGGTGGAAAA | IMR8545: AAAGTCGCTCTGAGTTGTTAT |
SRY-rev: TGATGGCATGTGGGTTCCTG | IMR8546: GGAGCGGGAGAAATGGATATG |