Table 2 Top 5 most significantly over-represented GO terms and KEGG pathways represented by annotated genes of transcripts differentially expressed in the ethanol, stress, or ethanol + stress groups compared to control (p < 0.01)
Ethanol | Stress | Ethanol + stress | |||
|---|---|---|---|---|---|
Term | p value | Term | p value | Term | p value |
Molecular function | |||||
 Haptoglobin binding | 1.64E−09 |  Protein binding | 8.30E−05 |  Haptoglobin binding | 6.68E−09 |
 Oxygen binding | 5.48E−07 |  Binding | 1.28E−04 |  Oxygen binding | 2.17E−06 |
 Hemoglobin alpha binding | 1.97E−06 |  Protein serine/threonine/tyrosine kinase activity | 0.001 |  Hemoglobin alpha binding | 4.56E−06 |
 Hemoglobin binding | 1.69E−05 |  Transferase activity | 0.002 |  Hemoglobin binding | 3.89E−05 |
 Protein binding | 1.84E−05 |  Catalytic activity, acting on a protein | 0.002 |  Oxygen carrier activity | 9.20E−05 |
Biological process | |||||
 Regulation of alternative mRNA splicing, via spliceosome | 3.75E−07 |  Microtubule cytoskeleton organization involved in establishment of planar polarity | 8.64E−05 |  Macromolecule modification | 2.34E−04 |
 Regulation of mRNA splicing, via spliceosome | 8.29E−07 |  Golgi organization | 4.85E−04 |  Response to chemical | 2.37E−04 |
 Alternative mRNA splicing, via spliceosome | 1.25E−06 |  Endomembrane system organization | 0.001 |  Response to temperature stimulus | 2.39E−04 |
 Regulation of RNA splicing | 8.49E−06 |  Embryo development | 0.001 |  Cellular protein modification process | 2.55E−04 |
 Regulation of mRNA processing | 1.01E−05 |  Organonitrogen compound metabolic process | 0.001 |  Protein modification process | 2.55E−04 |
Cellular component | |||||
 Hemoglobin complex | 1.64E−09 |  Intracellular part | 7.05E−05 |  Hemoglobin complex | 6.68E−09 |
 Haptoglobin-hemoglobin complex | 3.67E−09 |  Intracellular | 9.42E−05 |  Haptoglobin-hemoglobin complex | 1.49E−08 |
 Synapse | 2.09E−05 |  Organelle part | 1.43E−04 |  Intracellular part | 1.39E−04 |
 Synapse part | 2.86E−05 |  Intracellular organelle part | 3.46E−04 |  Intracellular | 2.07E−04 |
 Postsynapse | 0.001 |  Basal cortex | 0.001 |  Cell part | 0.001 |
KEGG pathway | |||||
 African trypanosomiasis | 6.90E−06 |  Circadian entrainment | 0.002 |  African trypanosomiasis | 2.66E−05 |
 Malaria | 3.50E−05 |  Retrograde endocannabinoid signaling | 0.009 |  Malaria | 1.31E−04 |
 Steroid biosynthesis | 0.01 |  Glycerophospholipid metabolism | 0.012 |  Mucin type O-glycan biosynthesis | 0.002 |
 Porphyrin and chlorophyll metabolism | 0.028 |  GABAergic synapse | 0.012 |  Glycine, serine, and threonine metabolism | 0.008 |
 ECM-receptor interaction | 0.029 |  Morphine addiction | 0.012 |  Systemic lupus erythematosus | 0.008 |