Fig. 2
Social behaviour assays reveal lack of genotypic difference between Atrx-cKO and control mice. a Total amount of social interaction with a mouse conspecific for genotype and sex during the 10-min social approach assay (twANOVA, genotype, F(1, 49) = 2.496, p = 0.121; twANOVA, sex, F(1, 49) = 4.086, *p = 0.048). b Social interaction over 1-min intervals during the social approach assay (twANOVA, genotype, F(1, 51) = 1.489, p = 0.228; twANOVA, sex, F(1, 51) = 3.425, p = 0.070). c Amount of time CtrlMALE (twANOVA, F(2, 32) = 78.39, ****p < 0.0001; mc, ****p < 0.0001) and Atrx-cKOMALE (twANOVA, F(2, 18) = 58.26, ****p < 0.0001; mc, ****p < 0.0001) spent in the empty centre chamber and chambers containing either a stranger mouse or novel object in the social preference assay. d Amount of time CtrlFEMALE (twANOVA, F(2, 24) = 31.39, ****p < 0.0001; mc, ****p < 0.0001) and Atrx-cKOFEMALE (twANOVA, F(2, 24) = 14.88, ****p < 0.0001; mc, *p < 0.05, ****p < 0.0001) spent in chambers containing either a stranger mouse or novel object, or the empty centre chamber e. Time spent in the empty centre chamber and chambers containing a familiar mouse or a novel mouse in the social novelty assay for e. CtrlMALE (twANOVA, F(2, 32) = 51.38, ****p < 0.0001; mc, ***p < 0.001, ****p < 0.0001) and Atrx-cKOMALE mice (twANOVA, F(2, 18) = 27.26, ****p < 0.0001; mc, ***p < 0.001, ****p < 0.0001), and f CtrlFEMALE (twANOVA, F(2, 24) = 11.77, ***p < 0.001; mc, ***p < 0.001) and Atrx-cKOFEMALE mice (twANOVA, F(2, 22) = 12.50, ***p < 0.001; mc, ***p < 0.001). g The sociability index for each mouse was calculated as the time spent in the stranger mouse chamber, divided by the total time in the stranger mouse and novel object chambers (twANOVA, genotype, F(1, 28) = 0.019, p = 0.890; twANOVA, sex, F(1, 48) = 3.574, p = 0.065). h The social memory index for each mouse was calculated as the time spent in the novel mouse chamber, divided by the total time in the familiar mouse and novel mouse chambers (twANOVA, genotype, F(1, 48) = 2.217, p = 0.143; twANOVA, sex, F(1, 48) = 5.811, *p = 0.019). Atrx-cKOMALE: n = 10, CtrlMALE: n = 17, Atrx-cKOFEMALE: n = 13, CtrlFEMALE: n = 13. Error bars: ±SEM