Fig. 3
From: Protective role of mirtazapine in adult female Mecp2+/− mice and patients with Rett syndrome
Analysis of the effects of treatment with MTZ (10 mg/kg) on parvalbumin-positive interneurons (PV+ INs) in 6-month-old Mecp2tm1.1Bird female mice. a–c Examples of PV and Hoechst staining of brain sections in the three regions of interest (ROIs) analyzed: the primary motor cortex, the barrel cortex, and the basolateral amygdala (BLA). (scale bar: 50 μm). d–f Density of PV+ INs in the three ROIs. g–i Mean intensity of PV+ INs’ somata within the three ROIs. All data are expressed as median ± interquartile range, n = 4–5 mice per group; 177–283 neurons per mouse brain (for cortical ROIs) or 38–88 neurons per mouse brain (for BSA). According to results of Saphiro-Wilk test, either one-way ANOVA, followed by Tukey’s post hoc test, or Kruskal-Wallis test, followed by corrected Dunn’s post hoc test was performed. Multiple selected comparisons comprehended: WT-VEH vs WT-MTZ, WT-VEH vs HET-VEH, and HET-VEH vs HET-MTZ. In order to determine an eventual genotype effect, 2-way ANOVA was performed (d and f). ns: p > 0.05 (not shown); *p ≤ 0.05; **p ≤ 0.01; ***p ≤ 0.001