Fig. 3
From: Deficits in higher visual area representations in a mouse model of Angelman syndrome
Two-photon population calcium imaging of stimulus-evoked responses in inhibitory and excitatory neurons. a ISOI is used to identify V1 and HVA boundaries as well as cortical areas that are responsive to the slow-fast gratings to create two sets of ROIs. Areas activated within V1 and AL were registered with respect to the vasculature. Scale bar, 1 mm. b Schematic of two-photon imaging setup. Stimuli are presented to the contralateral (left) eye via an optically isolated monitor. The vasculature map was used to target activated areas within V1 and AL. Sample FOV projection from V1 of a NexCre/+::Ai96Ds/+::Ube3am+/p+ mouse (Nex, excitatory neurons) and a Gad2Cre/+::Ai96Ds/+::Ube3am+/p+ mouse (Gad2, inhibitory interneurons). c Sample traces from excitatory neurons in V1 and AL of both WT and AS mice. Mean is plotted with SEM. d Sample traces from inhibitory interneurons in V1 and AL of both WT and AS mice. Mean is plotted with SEM