Fig. 3

AG490 upregulates MeCP2 in different cellular contexts. a qRT-PCR analysis of AG490 treatments in a humanized Xi-containing line (THX88) is shown. Dots indicate individual experimental points, samples are color-coded (see legend), and concentrations are indicated. ***Statistical significance, p ≤ 0.001, one-way ANOVA. The experiments were done using 2 (5-Aza), 3 (Jaki), and 4 (AG490) biological replicates, using 2 to 3 technical replicates per experiment. Horizontal lines displayed the median of the samples. Gapdh was used as an internal normalization control. b Survival analysis of H4SV cells in HAT-selecting medium in the presence or absence of Xi-reactivating compounds. Fold changes (FC, A.U) of the total number of colonies for control (DMSO) and Xi-reactivating drugs are shown. Bars indicate the standard deviation (SD). *Statistical significance, p ≤ 0.05. The average of three biological replicates are shown. Representative plates are shown below. c qRT-PCR data showing the effect of the indicated drug treatments on Mecp2, Hprt, and Xist mRNA levels; mean (± SD) are shown, *statistical significance, p ≤ 0.05 (n = 3 per compounds, the average of three biological replicates are shown)