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Fig. 2 | Journal of Neurodevelopmental Disorders

Fig. 2

From: Long-term spatial tracking of cells affected by environmental insults

Fig. 2

RFP reporter expression in the postnatal brain of reporter transgenic mice prenatally exposed to sodium arsenite or ethanol. a Experimental timeline. b Immunohistochemistry for RFP in the cerebral cortex and hypothalamus of the reporter transgenic mice (line A4) that were prenatally exposed to PBS (control), sodium arsenite, or ethanol (EtOH) as indicated. Results from 3 different animals each are shown for sodium arsenite- and ethanol-exposed groups. Broken lines indicate the pial surface. Sections were counterstained with DAPI. Observations were made at P20. c The average density of RFP+ cells (in coronal sections) throughout the cerebral cortex; F(2, 15) = 10.66, P = 0.001 by one-way ANOVA, **P < 0.01 by Tukey test. d The average density of RFP+ cells (in coronal sections) throughout the hypothalamus; F(2, 15) = 15.69, P = 0.0002 by one-way ANOVA, **P < 0.01 by Tukey test. e Higher magnification views of the boxed areas 1–4 in (b). Pial surface is oriented to the top. Reporter expression was observed in various cell types in the cerebral cortex, including glial (1), presumptive Cajal–Retzius cell (2), interneuron (3), and pyramidal neuron (4) (arrowheads). The broken line indicates the pial surface

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