Fig. 3

Tbr1 in PND60 cortex. Tbr1 immunohistochemistry in cortex of DE- and FA-exposed mice. Tbr1 is normally localized to neurons in layers II/II and V, as demonstrated in the Allen Brain Atlas [60]. (a, b) Representative images of Tbr1+ cells (red), counterstained with the nuclear stain DAPI (blue), from FA- and DE-exposed males. DE-exposed males showed a statistically significant decrease in Tbr1-positive cell density in bin 2 (cortical layer II/III) compared to FA-exposed males (c). No differences in Tbr1-positive cell distribution were observed with DE exposure in female mice (d). Results represent the mean (± SE) of 5 mice from different litters for each experimental group; 3-5 sections/mouse were examined (***p < 0.001; unpaired t test with Welch’s correction)