Fig. 4

Calbindin in PND60 cortex. Calbindin (Calb) immunohistochemistry in cortex of DE- and FA-exposed mice. Calb is a calcium-binding protein; Calb-expressing interneurons have been shown to localize in cortical layer II/III [5]. (a, b) Representative images of Calb-positive cells (green), counterstained with the nuclear stain DAPI (blue), from FA- and DE-exposed females. In DE-exposed females, a statistically significant increase in Calb-positive cell density was found in bins 9-10 (cortical layer VI) when compared to FA-exposed females (c, d). No significant differences in Calb-positive cell distribution were found between FA- and DE-exposed male mice (c, d). Results represent the mean (± SE) of 5 mice from different litters for each experimental group; 3-5 sections/mouse were examined (*p < 0.05, **p < 0.01; unpaired T test with Welch’s correction)