Summary of samples for RT-qPCR (all ages) | |||||||
Sample | N | Median age | male | female | Region of brain | Mutation | |
Postnatal controls | 27 | 85 months | 12 | 15 | front - 14, temp - 8, par - 1, cortex - 4 | N/A | N/A |
Cortical tubers | 35 | 115 months | 17 | 19 | front - 26, temp - 9 | TSC1 - 9 | TSC2 - 26 |
Samples for WB (young TSC vs control) | |||||||
Postnatal controls | Age | Gender | Region of brain | Mutation | |||
WB CTRL1 | 6 weeks | m | front | – | |||
WB CTRL2 | 7 weeks | f | front | – | |||
WB CTRL3 | 4 months | m | front | – | |||
WB CTRL4 | 7 months | f | cortex | – | |||
WB CTRL5 | 1 year | f | temp | – | |||
WB CTRL6 | 2 years | f | temp | – | |||
WB CTRL7 | 3 years | m | front | – | |||
Resected cortical tubers | Age | Gender | Region of brain | Mutation | |||
WB TSC1 | 8 months | m | temp | TSC2 | |||
WB TSC2 | 1 year | m | front | TSC2 | |||
WB TSC3 | 2 years | m | front | TSC2 | |||
WB TSC4 | 2 years | m | front | TSC2 | |||
WB TSC5 | 2 years | f | front | TSC1 | |||
WB TSC6 | 3 years | f | temp | TSC2 | |||
WB TSC7 | 3 years | m | front | TSC2 | |||
Samples for immunohistochemistry | |||||||
Postnatal controls | Age | Gender | Region of brain | Mutation | |||
IHC CTRL1 | 2 years | f | front | – | |||
IHC CTRL2 | 2 years | m | front | – | |||
IHC CTRL3 | 10 years | m | front | – | |||
IHC CTRL4 | 15 years | m | front | – | |||
Resected cortical tubers | Age | Gender | Region of brain | Mutation | |||
IHC TSC1 | 2 years | m | front | TSC2 | |||
IHC TSC2 | 4 years | f | front | TSC1 | |||
IHC TSC3 | 9 years | m | front | TSC2 | |||
IHC TSC4 | 13 years | f | front | TSC2 | |||
IHC TSC5 | 16 years | m | front | TSC2 |