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Fig. 4 | Journal of Neurodevelopmental Disorders

Fig. 4

From: LiCl treatment leads to long-term restoration of spine maturation and synaptogenesis in adult Tbr1 mutants

Fig. 4

LiCl rescues inhibitory synapse deficits in mPFCx of Tbr1layer5 CKOs. A Representative traces of miniature inhibitory postsynaptic currents (mIPSCs) from layer 5 mPFCx neurons of P65-106 Tbr1wildtype (black) and Tbr1layer5CKO (cyan) mice following saline administration (4 ml/kg) at P30. Scale bars: 20 pA, 100 ms. B Left: quantification of mIPSC frequency. Mann-Whitney ***p = 0.0005 (Tbr1wildtype+saline: 15.6 ± 1.0 Hz, n = 18, Tbr1layer5CKO+saline 9.4 ± 1.2 Hz, n = 19). Right: cumulative probability distribution of mIPSC inter-event intervals. Kolmogorov-Smirnov ****p < 0.0001. C Left: quantification of mIPSC amplitude. Mann-Whitney p = 0.32 (Tbr1wildtype+saline: 9.24 ± 0.6 pA, n = 18, Tbr1layer5CKO+saline: 9.08 ± 0.5 pA, n = 19). Right: cumulative probability distribution of mIPSC amplitude. Kolmogorov-Smirnov *p = 0.04. D Representative traces of mIPSCs from layer 5 neurons of LiCl (400 mg/kg) treated P95-104 Tbr1wildtype (green) and Tbr1layer5CKO (purple) mice. Scale bars: 20 pA, 100 ms. E Left: quantification of mIPSC frequency. Mann-Whitney p = 0.77 (Tbr1wildtype+ LiCl: 15.84 ± 1.7 Hz, n = 14, Tbr1layer5CKO+ LiCl: 18.73 ± 2.7 Hz, n = 11). Right: cumulative probability distribution of mIPSC inter-event intervals. Kolmogorov-Smirnov p = 0.47. F Left: quantification of mIPSC amplitude. Mann-Whitney p = 0.57 (Tbr1wildtype+ LiCl: 8.6 ± 0.4 pA, n = 14, Tbr1layer5CKO+ LiCl 9.1 ± 0.8 pA, n = 11). Right: cumulative probability distribution of mIPSC inter-event intervals. Kolmogorov-Smirnov p = 0.86. Boxplots are min to max show all points

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