Fig. 4

RCAN1 knockout and overexpression bidirectionally perturb wheel running patterns of free-running young mice. A Mean total (CT0-CT24) daily wheel running of free-running young mice. Rcan1 KO mice exhibit increased total daily wheel running compared with Rcan1 WT and RCAN1 TG mice, while RCAN1 TG mice displayed decreased total daily wheel running compared with NTG mice. B Mean daily alpha (active) phase wheel running of free-running young mice in DD. Rcan1 KO mice show increased daily wheel running in the alpha phase compared with Rcan1 WT and RCAN1 TG mice, while RCAN1 TG mice show decreased daily wheel running in the alpha phase relative to NTG mice. C Mean daily rho (inactive) phase wheel running of free-running young mice in DD. Rcan1 KO mice exhibit elevated daily wheel running during the rho phase compared with Rcan1 WT and RCAN1 TG mice. D Mean percentage of total daily wheel running during the rho phase for free-running young mice. Rcan1 KO mice display an increased percentage of total daily wheel running in the rho phase compared with Rcan1 WT mice, while RCAN1 TG mice trended toward an increased percentage of total daily activity in the rho phase compared to NTG mice. N = 17 Rcan1 WT, 21 Rcan1 KO, 17 NTG, 18 RCAN1 TG mice. All data are mean ± S.E.M. *p < 0.05; **p < 0.01; ****p < 0.0001