Fig. 2
From: Focus on your locus with a massively parallel reporter assay
Luciferase assay vs cis-regulatory MPRA. A Luciferase assay measures light emitted by a reporter gene, luciferase, driven by a regulatory element. B In a canonical cis-regulatory MPRA, the regulatory element drives the RNA expression of the unique barcodes. Transcriptional activity is quantified as barcode transcription (via RNA-seq of the barcodes) normalized to initial input of barcodes (via DNA-seq of the barcodes). Thousands of cis-regulatory elements (CREs) can be tested in the same experiment