The feasibility and utility of hair follicle sampling to measure FMRP and FMR1 mRNA in children with or without fragile X syndrome: a pilot study

Table 4 FMR1 mRNA Levels by Collection Type and Collection Location – Descriptive Statistics

Notes: For the hair follicle and buccal swab collection types, the mean FMR1 mRNA Delta Cq value across all samples for each subject is summarized
For the blood collection type, two Relative FMR1 values were reported for Subjects 101-002, 101-005, and 101-007; only the smallest value for each subject is summarized
Buccal and hair follicle FMR1 mRNA were quantified by qRT-PCR and blood FMR1 was measured by PrimeFlow^TM. The 2 measurements cannot be directly compared across sample types. Within sample type comparisons between healthy participants and FXS participants are appropriate
For FMR1 PrimeFlow^TM values are reported as FMRP/IC (dapB) Median Fluorescent Intensity (MFI)
Abbreviations: BLQ below limit of quantification, Max maximum, Min minimum, n number of subjects, SD standard deviation

ISSN: 1866-1955