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Fig. 5 | Journal of Neurodevelopmental Disorders

Fig. 5

From: NRXN1 depletion in the medial prefrontal cortex induces anxiety-like behaviors and abnormal social phenotypes along with impaired neurite outgrowth in rat

Fig. 5

Immunoblotting validation of key proteins centering on cell membrane structure. A Automated capillary Western blot validation of ANXA1, GRB2, and ANXA4. B The expression of ANXA1 (t = 14.216, df = 4, P < 0.001), GRB2 (t = 10.805, df = 4, P < 0.001), and ANXA4 (t = 10.118, df = 4, P = 0.009) was quantified according to the corresponding bands. GAPDH was used as the internal reference marker and loading control for normalization. These data were presented as mean ± SD of three independent experiments. NS > 0.05, **P < 0.01 and ***P < 0.001, compared with the corresponding control group

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