Visuospatial working memory in children and adolescents with 22q11.2 deletion syndrome; an fMRI study

22q11.2 deletion syndrome (22q11DS) is a genetic disorder associated with a microdeletion of chromosome 22q11. In addition to high rates of neuropsychiatric disorders such as schizophrenia and attention deficit hyperactivity disorder, children with 22q11DS have a specific neuropsychological profile with particular deficits in visuospatial and working memory. However, the neurobiological substrate underlying these deficits is poorly understood. We investigated brain function during a visuospatial working memory (SWM) task in eight children with 22q11DS and 13 healthy controls, using fMRI. Both groups showed task-related activation in dorsolateral prefrontal cortex (DLPFC) and bilateral parietal association cortices. Controls activated parietal and occipital regions significantly more than those with 22q11DS but there was no significant between-group difference in DLPFC. In addition, while controls had a significant age-related increase in the activation of posterior brain regions and an age-related decrease in anterior regions, the 22q11DS children showed the opposite pattern. Genetically determined differences in the development of specific brain systems may underpin the cognitive deficits in 22q11DS, and may contribute to the later development of neuropsychiatric disorders.

gated brain function during a visuospatial working memory (SWM) task in eight children with 22q11DS and 13 healthy controls, using fMRI. Both groups showed task-related activation in dorsolateral prefrontal cortex (DLPFC) and bilateral parietal association cortices. Controls activated parietal and occipital regions significantly more than those with 22q11DS but there was no significant between-group difference in DLPFC. In addition, while controls had a significant age-related increase in the activation of posterior brain regions and an age-related decrease in anterior regions, Introduction 22q11.2 deletion syndrome (22q11DS), also known as velo-cardio-facial syndrome (VCFS) is a genetic disorder associated with a microdeletion in chromosome 22q11.2 [1]. While the physical phenotype is variable, commonly reported features include characteristic dysmorphology, congenital heart disease and cleft palate [2,3]. A characteristic behavioural phenotype in 22q11DS has also been described with high rates of schizophrenia and attention deficit / hyperactivity disorder (ADHD) [4][5][6][7]. In addition, children and adults with 22q11DS typically have mild learning disabilities and a specific cognitive profile with particular deficits of visuospatial function, working memory, attention, executive function [8][9][10][11][12][13].
There is increasing evidence for a robust association between 22q11DS and schizophrenia [14]. Indeed, deletion of chromosome 22q11 represents the most commonly known risk factor for the development of schizophrenia and offers a unique model for identifying the neurobiological substrate underlying the development of psychotic disorders. In the general population, people with schizophrenia have deficits in a number of cognitive domains, including visuospatial working memory (SWM) [15][16][17][18][19][20][21][22][23][24][25][26]. Furthermore, recent neuropsychological and neuroimaging studies indicate that those at high-risk of developing psychosis also show deficits in SWM [15,[27][28][29][30][31]. For instance, Saperstein and colleagues compared the relative risk ratios for SWM deficits among 23 schizophrenics, 17 relatives of patients with schizophrenia with schizophrenia spectrum personality disorders (SSPD), 23 non-SSPD relatives of patients with schizophrenia, 14 SSPD commu-nity members with no family history of psychosis, and 36 healthy community controls [27]. Relative risk of SWM deficits, as compared with healthy community controls, increased with the relative genetic liability, where it was highest among patients with schizophrenia, second highest among SSPD family members, then family non-SSPD and community SSPD. The authors suggested that the pattern of SWM performance deficits reflected the proposed model of latent genetic liability, upholding SWM as a viable cognitive endophenotype [27]. Similarly, 22q11DS adults with schizophrenia are significantly more impaired in a number of cognitive domains, including spatial working memory (SWM), as compared to those without schizophrenia [12].
The neural basis for SWM has been investigated in many neuroimaging studies within the general population. These reported that SWM tasks activate a cortical pathway extending bilaterally from prefrontal cortex (more dorsal than ventral for spatial rather than object working memory) to anterior cingulate and parietal regions. This is true in both children and adults [32][33][34][35]. Furthermore, relatively few studies that have also examined the developmental changes in brain functions during normal brain development reported that there may be an age-related increase in brain activations in frontal and parietal cortical areas [32,33,36], which is consistent with developmental changes in the brain structure [37].
Frontal cortex may be functionally different in people with schizophrenia. For example, many neuroimaging studies on working memory processing in schizophrenia report aberrant activation of prefrontal cortex although changes have not always been consistent [20,25,[38][39][40][41][42][43]. In addition, a recent fMRI study of brain function during a memory-guided saccade SWM task in the offspring of people with schizophrenia (i.e. a 'High Risk' group) reported that they also had significantly reduced activation of the DLPFC and parietal cortex as compared to controls [29]. Thus, there is preliminary evidence that abnormalities in the function of frontal, and perhaps parietal, cortex during SWM tasks may precede the onset of schizophrenia.
We, and others, previously reported that people with 22q11DS have neurodevelopmental abnormalities in the anatomy of frontal and parietal regions [44][45][46][47][48] and possibly a disruption in the connectivity of the frontalparietal pathway [49,50]. As noted above, in the general population, aberrant activation of these brain regions during SWM has been reported in people at risk of developing psychosis [29]. However, to date nobody has examined brain function during a SWM task in people with 22q11DS, or examined if the functional developmental trajectory of these regions is different. Thus, we (1) investigated the neural functioning in children and adolescents with 22q11DS and healthy controls during a SWM task, and (2) carried out a preliminary analysis of age-related differences in brain function.

Subjects
We included 21 volunteers who could reliably carry out the SWM task (eight subjects with 22q11DS and 13 healthy controls). All were right-handed. The 22q11DS group comprised four males and four females, aged 9-16 years and the healthy controls included eight males and five females, aged 8-17 years (see Table 1 for demographic data). Where possible we used sibling-controls (eight out of 13) in order to match as best we could for socioeconomic factors. The sibling and non-sibling controls did not significantly differ in FSIQ (F<1).
The 22q11DS volunteers were recruited through the 22q11 (UK) Support group and the Behavioural Genetics Clinic at the Maudsley Hospital/Institute of Psychiatry, Kings College London. 22q11DS was diagnosed by fluorescence in-situ hybridisation (FISH) using the N25 probe (Oncor Inc). Eight of the control group were siblings, and five were recruited from the local community. All the participants, and/or their parents gave written informed consent/assent as approved by the local research ethics committee (Institute of Psychiatry, South London and Maudsley Trust).
All subjects underwent routine psychiatric and physical examination, and routine blood tests. Overall cognitive ability was measured using the Wechsler Intelligence Scale for Children-III (WISC-III) [51]. We excluded people with a clinically detectable co-morbid psychiatric disorder or physical disorder affecting brain function (e.g. ADHD, hypothyroidism, or epilepsy), or with a clinically abnormal MRI scan-as determined by a neuroradiologist. As expected controls had significantly higher scores for performance IQ (PIQ), verbal IQ (VIQ), and full scale IQ (FSIQ) (p<.001). All were drug free at the time of testing.

Tasks
We used a Spatial Working Memory (SWM) 'blockdesign' task, based on one developed by Klingberg et al. [32]. In our version, ten brown, square boxes (see Fig. 1) in random positions, instead of the original 4×4 matrix, were used as possible target locations in order to minimize the possibility of giving verbal labels for each location. The locations of the boxes were changed from trial to trial.
Each trial began with the presentation of ten boxes for 500 ms to indicate the possible target locations for the trial. A picture of an apple (target) appeared sequentially in some of these boxes for 1,000 ms. Either three or five of the boxes per trial were used as target locations, creating two conditions (Fig. 2). The gaps between the presentations of the target locations were 2,000 and 500 ms respectively for the three and five apple conditions. This kept the overall presentation time constant at 7,000 ms. After 2,000 ms following the last target location, one of the boxes went blue for another 2,000 ms. The subjects had to press one of the two buttons to indicate whether or not this box was a target location during this period. Then, the screen became blank for 1,000 before the next trial began. Half the subjects were instructed to press the left button if the blue box was in one of the target locations and the right button for the non-target locations. For the other subjects, the response-to-button mapping was reversed. For button responses, the subjects used a rectangular box (6×11 cm) where two square buttons (1 cm 2 ) were placed in the middle with a gap of about 2.5 cm. They were instructed to hold the box with their left hand and press the buttons with their right index and middle fingers.
There was also a baseline task in which the boxes were arranged in the shape of an "X" (see Fig. 1), with the target locations appearing in the four corner boxes in a predictable order. Subjects were instructed to look at the  target as it appears in different locations, and then press a pre-specified button when one of the boxes in the middle was highlighted in blue. This way, both tasks had the components of visual stimulation, eye movements and motor output, while the SWM task had an extra component of SWM. There were two runs of ten blocks each where the subjects alternated between the baseline block and SWM block (10 blocks×baseline; 5 blocks×swm3; 5 blocks× swm5 in total). There were three trials of the same condition (i.e. baseline, swm3, swm5) in each block, and a brief message at the beginning of each block indicated whether it was to be a baseline block or a SWM block. The two conditions of the SWM task (i.e. three-targets or five targets) were distributed in pseudo-random order.

Practice
Prior to the scan, all the subjects completed an offline version of the SWM task to practise the task. The subjects were first given a block of ten SWM trials with just two target locations. This was repeated until the subjects could respond with an accuracy rate of more than 80%. Then, the subjects completed the eight blocks of ten SWM trials. The number of target locations for each block increased from two to eight. The target stimuli were presented at the fixed rate of 1,000 ms presentation time and 1,000 ms interval. For each trial, an auditory feedback (a high or low tone) was given to indicate whether or not the response made was correct. After completing the offline task, the subjects were given four blocks of the scanning version of the task to get familiarised with the setting.

Image acquisition
All functional imaging data were acquired at 1.5 T using a GE, Neuro-optimised MR System (General Electric Healthcare, Milwaukee, WI) based at the Maudsley Hospital, London. A quadrature birdcage headcoil was used for radio frequency transmission and reception. An inversion recovery echoplanar imaging (EPI) dataset was acquired at 43 near-axial 3 mm thick planes parallel to the anterior commissure-posterior commissure (AC-PC) line: time-toecho (TE) 73 ms, inversion time (TI) 180 ms, repetition time (TR) 16 s, in-plane resolution 1.72 mm, interslice gap 0.3 mm, matrix size: 128×128 pixels. This higher resolution EPI dataset provided whole brain coverage and was later used to register the fMRI datasets acquired from each individual in standard stereotactic space. 16 near-axial noncontiguous slices (7-mm thick, 0.7-mm slice skip) were prescribed parallel to the anterior commissure-posterior commissure (AC-PC) line and covering the whole brain: TE 40 ms, TR 2 s, in-plane resolution 3.44 mm, interslice gap .7 mm, matrix size: 64×64 pixels.
For each experimental series, 220 T2*-weighted, echoplanar images depicting Blood Oxygenation Level Depen- , subjects had to remember the positions of the boxes in which an apple (target) was presented sequentially. After 2,000 ms of delay following the presentation of the target in the final box for a given trial, one of the boxes was highlighted, and the subjects pressed a button to indicate whether or not the target had appeared in this box.
For the "memory load=3" condition, the target was presented in three of the ten boxes, whereas for the "memory load=5" condition, five boxes were used target locations. For the baseline task (bottom), the stimuli were presented sequentially in the predictable order (i.e. topleft, top-right, bottom-left, bottom-right). When the middle box was highlighted after the delay, subjects pressed the pre-specified button dent (BOLD) contrast [52] were collected from each slice at an echo time (TE) of 40 ms and a repetition time (TR) of 2 s. Four dummy acquisitions were acquired at the beginning of each series to ensure that the spin system had reached steady state and the chosen flip angle was 70°and number of averages (NEX)=1.
Visual Stimuli were presented via a conventional PC and LCD projector system to a screen placed at the feet of the subject and overt responses were made using an MR compatible two-button box placed in the subject's dominant right hand. Presentation of all stimuli and recording of all subject responses were synchronised to the imaging system. The total functional image acquisition time for each series was 7 min 28 s.

Individual brain activation maps
Data were analysed using a non-parametric approach with XBAM, the local software developed at the Institute of Psychiatry [53]. The images were first processed to minimize motion related artefacts [54]. They were then smoothed using a Gaussian filter (FWHM 7.2 mm) to improve the signal to noise characteristics of the images.
Responses to the experimental paradigms were then detected by first convolving each component of the experimental design with each of two gamma variate functions (peak responses at 4 and 8 s respectively). The best fit between the weighted sum of these convolutions and the time series at each voxel was computed using the constrained BOLD effect model suggested by Friman et al. [55]. A goodness of fit statistic was computed, consisting of the ratio of the sum of squares of deviations from the mean image intensity (over the whole time series) due to the model to the sum of squares of deviations due to the residuals (SSQratio).
The data were then permuted by the wavelet-based method described and extensively characterized in Bullmore et al. [56]. Using this distribution, it is possible to calculate the critical value of SSQratio needed to threshold the maps at any desired type I error rate. The detection of activated voxels is extended from voxel to cluster level using the method described in detail by Bullmore et al. [57].

Group brain activation maps
The observed and randomised data for each individual are transformed into the standard Talairach space [58] using the procedure described in detail in Brammer et al. [53]. Group activation maps are then computed by determining the median SSQratio at each voxel (over all individuals) in the observed and permuted data maps (medians are used to minimize outlier effects). Analysis was extended from voxel to cluster level by the permutation method described by Bullmore et al. [57] and the expectation false positive clusters set to <1 per brain.

Between group analysis
Group differences were estimated on a voxel-wise basis across the whole brain by fitting a regression model of the type to the data at each voxel. In this formulation, y represents a vector of individual subject SSQratios at each voxel in standard space, X is a group contrast matrix and e is a vector of residual errors at each voxel. The estimate of the parameter b describes the magnitude of the group difference. The significance of these estimates of b is determined by permutation as follows. The elements of the contrast matrix X are permuted to give a random assignment of subjects to each group and the model refitted. Repeating this procedure a large number of times at each voxel and subsequently combining the estimates of b over all voxels produces the distribution of b under the null hypothesis of no effect of group membership on activation. Observed values of b in the unpermuted data can then be tested against this distribution to assess their probability under the null hypothesis. This analysis is also extended to the cluster level as previously described (Bullmore et al. [54]).

Correlations of age and brain activities
We also conducted a correlation analysis to examine agerelated differences in brain functions associated with SWM in 22q11DS and controls, then contrasted the trajectories between the groups. Correlational analysis is carried out by computing the Pearson product-moment correlation coefficient at every voxel between the subject ages and statistical values (SSQ ratios). The significance of this correlation at whole brain level is determined by permuting the order of subjects 50 times at each voxel and re-determining the correlation coefficient. Combining the correlation coefficients following permutation over the whole brain gives us the null distribution of correlation coefficients. The significance of the observed (pre-permutation) correlation coefficients is then determined at voxel and cluster level using the data-driven null distribution produced as described above.

Group comparison of age correlations
In order to compare the age-correlation (i.e. developmental differences) between the groups, the Pearson productmoment correlation coefficient was calculated for each group independently and the difference between the correlation coefficients was then computed. To test the significance of this difference, the behavioural data were randomly permuted between the two groups and the above calculation procedure repeated. The permutation/calculation procedure was repeated 50 times at each voxel and the resulting data combined over all voxels to form a "global" distribution of correlation coefficient differences under the null hypothesis of no difference between the correlation between behaviour and BOLD response in the two groups. The significance of any observed difference in correlation in the original data could then be assessed by reference to this distribution.

Task performance
For each subject, task performance was assessed as the percentage of correct responses for the SWM task. Since the accuracy levels for the SWM tasks and the baseline task did not significantly differ between the sibling and nonsibling controls, and neither of them have a deletion in 22q11.2, data from both sibling and non-sibling healthy children have been analysed together as the healthy control group. The task performance for the 22q11DS group was 73(±16)% and 79(±12)% for the target=3 and target=5 conditions, respectively, whereas, these were 88(±10)% and 88(±10)% in the healthy control group. The accuracy level for the baseline task was 97(±5)% for the 22q11DS group and 96(±6)% for the control group. An analysis of variance (ANOVA) (group × task type) on the accuracy revealed there was a significant main effect of group (F(1, 57)=8.0, p<0.01); the main effect of task type (F(2, 57)=13.2, p<0.001); but the interaction was not significant (F(2, 57)= 2.9, p=0.06). The main effect of group indicated that the overall performance accuracy was significantly higher in the controls. A post-hoc analysis suggested that in both groups the accuracy rates for the baseline task was higher than those for the SWM tasks. There was no significant effect of memory load (swm3 and swm5) on performance accuracy.

Image data analysis
Since there was no difference in brain activations between the target=3 and target=5 conditions data from the two conditions were combined in order to increase statistical power. An averaged activation map image (voxel p=0.05; cluster p=0.01) was created for each group (see Fig. 3). At this level of stringency there was less than one false positive cluster for each analysis. Table 2 shows the areas activated significantly more during the SWM task than during the baseline task for each group.

Group comparison
Both groups significantly activated brain regions normally associated with SWM processes, such as, DLPFC, parietal regions, as well as primary visual regions in the occipital lobule ( Fig. 4 and Table 3). We compared group activation using an analysis of variance (voxel p=0.01; cluster p= 0.01) on the "SWM activation-baseline activation" data. At this level of stringency there was less than one false positive cluster for analysis. Controls had significantly greater activation than people with 22q11DS (Table 3) bilaterally in precuneus (BA 7, 31), and right cuneus (BA 18). In order to see if differences in parietal activation could have been related to significant differences in task performance, we have carried out correlation analysis between task performance accuracy and brain activities. (see Fig. 4). Significantly positive correlation between task performance and parietal activities was found in the left precuneus (BA 7) in the 22q11DS group but absent in the controls. In contrast, significantly negative correlations was found in the left inferior parietal lobule (BA 40) in the controls. Thus, it was unlikely that the significant group differences in the parietal region were attributable to higher performance in the controls. Indeed, the overall pattern of group differences remained even when we re-ran the same   Fig. 4 and Table 4).
Decreased activation with age There was also a significant negative correlation (voxel p=0.005; cluster p=0.01; false positive cluster <1) between age and decreased brain activations. For the healthy controls, most of the agerelated decrease was observed in frontal areas (e.g. bilateral VLPFC, middle frontal gyri, left DLPFC, and right precentral gyrus). In contrast, the 22q11DS group showed an age-related decrease mostly in non-frontal areas, such as bilateral parietal regions, fusiform gyri, cingulate gyri, right temporal regions and parahippocampal gyrus. (see Fig. 4 and Table 5).
Group differences in age effect There were significant differences between the 22q11DS and control groups in the developmental trajectories of brain activations for SWM (see Table 6). Fig. 4 Areas that showed significant correlation between task performance and brain activations were observed (voxel p=0.05, cluster p=0.01) Overall, the controls showed significantly larger age-related changes in brain activations (both increase and decrease) than 22q11DS in widespread cortical and subcortical regions, predominantly in the right hemisphere (e.g. middle frontal gyrus (BA 25), inferior parietal lobule (BA 40), superior temporal gyrus (BA 42), parahippocampal gyrus (BA 36), hippocampus, caudate, and brain stem), as well as bilateral inferior frontal gyrus (BA11), and left thalamus and cerebellum. The 22q11DS group, in contrast, showed larger correlational changes than the controls in the left inferior frontal gyrus (BA 44), and caudate only.

Discussion
We investigated brain function during a SWM task in children and adolescents with 22q11DS, and healthy controls. Both groups showed activation of the DLPFC, parietal and occipital regions during performance of the visual SWM task. However, healthy controls had significantly greater activation than children with 22q11DS, in parietal and occipital regions with no significant difference in activation of frontal regions between both groups. Fig. 5 Areas that showed age-related change in brain activation were observed (voxel p=0.005; cluster p=0.01) Several prior studies have reported that people with 22q11DS have differences in the anatomy of frontal regions [44][45][46][47][48]. In addition, people with schizophrenia and non-22q11DS 'high risk' groups are reported to have significant differences in activation of the DLPFC and parietal cortex [29]. Hence, we anticipated to find functional differences in these brain regions in people with 22q11DS (who are also at risk for developing schizophrenia). In agreement with Keshavan et al., [29], we found a significant difference in the activation of parietal regions, but not DLPFC. In an fMRI study on 22q11DS employing a task that requires frontal and parietal activation (i.e. arithmetic task), Eliez and colleagues [59] also found significant differences in the parietal, but no difference in the prefrontal region, suggesting that the findings from both studies are consistent.
Absence of group differences in PFC may simply mean that they could not be detected because of the relatively small sample size in the 22q11DS group (n=8) with 1.5 T magnet in the current study, as well as in the study by Eliez et al. [59]. However, potential biological explanations include genetically determined differences in brain anatomy. Significant differences in the anatomy of various brain regions have been reported in young people with 22q11DS. For instance, recent imaging studies report that 22q11DS children and adolescents have a significantly reduced volume of grey matter in the parietal lobe, but a proportionally larger frontal lobe [44,45]. Hence, reduced parietal activation may be explained by a regionally specific reduction in brain volume. In contrast, we may have found no difference in activation of DLPFC because the frontal structures may be relatively preserved, and hence, people Minimum cluster size=5 voxels with 22q11DS become increasingly dependent on this region as previously suggested [59]. Alternatively, disruption in the 'connectivity' within the cortical network which underpins SWM, may explain our results. For example, in the healthy population DLPFC and parietal regions are activated during SWM tasks [60][61][62]. Also, Barnea-Goraly and colleagues [49] recently reported that people with 22q11DS have disrupted 'connectivity' (as measured using diffusion tensor imaging (DTI)) between frontal, parietal, and temporal regions. Thus, our finding of reduced activation in parietal regions may be explained by disrupted parietal 'connectivity'. In order to address this issue directly we plan further DTI studies in these subjects.
There is also a potential genetic explanation for the absence of significant differences in frontal activation between 22q11DS people and controls. Recent studies suggest that the gene coding for catechol-O-methyltransferase (COMT), which maps to the deleted region of 22q11 in 22q11DS, has neurobiological effects on the prefrontal cortex [63][64][65][66][67][68][69][70]. Egan and colleagues [70] reported that the Val (high-activity) COMT allele is associated with impaired prefrontal cortical function in both healthy populations and people with schizophrenia, while additional studies have reported that the low-activity Met allele is associated with superior prefrontal cortical function in both groups [69][70][71]. However, the effect of the low activity Met allele in people in 22q11DS remains unclear. There has been evidence that 22q11DS people with the Met allele perform significantly better [72] than those with the Val allele on executive function tasks which activate prefrontal regions (i.e. set-shifting, verbal fluency, attention, and working memory). Conversely, Gothelf and colleagues [73] reported that the low-activity Met allele is a risk factor for both the decline in prefrontal cortical volume and the consequent development of psychotic symptoms during adolescence in people with 22q11DS. Our current sample size is too small (22q11DS subjects with Met allele=3; those with Val allele=5) to examine the possible effect of the COMT Val 108 / 158 Met polymorphism in 22q11DS, but we plan to address this issue in a future study. Our study has some limitations. For example, the sample size was relatively small, which may account for the absence of group differences in the DLPFC. In addition, the controls were significantly more intelligent than people with 22q11DS, and the accuracy rate in the control group was approximately 20% higher than that in the 22q11DS group. Hence, it may be suggested that our results may be explained simply by differences in ability to perform the task, and/or it might have been better to have included an IQ matched non-deleted control group who were also matched on social and environmental factors. However, we compared people with 22q11DS to healthy controls (siblings of the 22q11DS subjects, where available) because our question was how brain activation in people with 22q11DS differs from the general population. Also, both groups activated brain regions which have been reported by others to be specifically implicated in SWM processing in both children and adults [32][33][34][35]. Hence, we were able to determine that our task was valid. In addition, the between-group differences remained even when we covaried for accuracy rate. Furthermore, unrelated learning disabled controls would most likely have comprised a heterogeneous population with a large variety of genetic and environmental/social differences. Hence, where possible we used sibling controls to account, as best we could, for environmental and social differences. Thus, the signifi- cantly higher activations in the healthy control group probably cannot be solely attributed to the better task performance or environmental and/or socio-economic factors.
In addition to the group differences in brain activation patterns, we also found preliminary evidence that people with 22q11DS may have differences in the developmental trajectories of brain function during SWM tasks. Within the healthy control group, an age-related increase in brain activations was observed largely in the posterior regions (e.g. bilateral superior parietal lobule, right angular gyrus, left inferior parietal lobule, precuneus, fusiform gyrus, inferior temporal gyrus, inferior-middle occipital gyrus) and a decrease mostly in the anterior regions (e.g. bilateral VLPFC, middle frontal gyri, left DLPFC, right precentral gyrus). In contrast, people with 22q11DS people tended to use more anterior (e.g. left VLPFC), and less posterior (e.g. bilateral precuneus, fusiform gyri, cingulate gyri), regions as they grow older. The age-related increases we found in the frontal and parietal cortical regions of controls are similar to findings from previous studies in normally developing children [32,33]. Furthermore, a preliminary analysis of between-group differences in ageing indicated that there are significant differences between the developmental trajectories in the brain functions associated with SWM. Thus, our findings may provide preliminary evidence that, as people with 22q11DS develop, they do not undergo the normal age-related 'shift' to activating more posterior brain regions during SWM tasks, and instead continue to activate more anterior (frontal) systems. The biological basis of this putative difference in post-natal brain maturation is unknown.
In summary, we examined brain function in children with 22q11DS during a visuospatial working memory task. Both groups activated brain regions classically implicated in carrying out the task. However, healthy controls had significantly greater activation than children with 22q11DS of parietal and occipital regions. In addition, we found no significant between-group difference in activation of frontal regions. The neurobiological basis for our findings is unknown, but we suggest that it may reflect neurodevelopment differences in brain anatomy and connectivity. Further work is required, and in larger samples, to examine this issue.