Fig. 2

THIP administration alleviated the breathing abnormalities in MECP2-null mice. A ₁ , A ₂ Typical records of breathing activity from both WT and MECP2-null mice with and without THIP administration. B Distributions of apnea count in different aged MECP2-null mice with and without THIP treatment. C In MECP2-null mice, THIP administration significantly reduced the apnea count at ages of 4–6 weeks (vehicle: n = 26, THIP: n = 8, P = 0.002) and 6–8 weeks (vehicle: n = 19, THIP: n = 8, P = 0.021), although the significance was not found in 2–4 weeks (vehicle: n = 45, THIP: n = 7, P = 0.081; ^### P < 0.001 in Kruskal-Wallis test; *P < 0.05, **P < 0.01 in Mann-Whitney post hoc comparison). D, E Similar effects of THIP treatment on breathing frequency variation was observed in these mice (2–4 weeks: P = 0.037; 4–6 weeks: P = 0.004; 6–8 weeks: P < 0.001; *P < 0.05, **P < 0.01, ***P < 0.001; one-way ANOVA and Tukey’s post hoc)