Fig. 2

a Expression profile of miR-137 in mouse primary hippocampal cultures over 11 days in vitro (DIV). The graph shows data relative to DIV3 (n = 3). b Luciferase assay in primary mouse hippocampal neurons treated with hsa-miR-137 or control power inhibitors at DIV5. Luciferase reporter constructs were transfected at DIV6, and luciferase activity was measured after 48 h. c Western blot with lysate from primary mouse hippocampal neurons treated with hsa-miR-137 or control power inhibitor at DIV5 and protein was harvested after 5 days. Cropped pictures indicate Shank2 and βIII-tubulin expression, and the full length blots are presented in Additional file 1: Figure S4B. Bar plots show mean ± SEM; *P < 0.05 two-way ANOVA; (n = 5 experiments)