Fig. 2

Microglia/macrophages are increased in the IUGR brain at P14. Sections of the IUGR and control brain at P14 were labeled with antibody to the microglia/macrophages marker IBA1 or activated microglia/macrophages marker CD68 as well as DAPI to identify nuclei. a Shown are sections of the corpus callosum from control and IUGR rat brains labeled with IBA1 (green) and DAPI (blue) at PD14 at × 10 and × 40 magnification (size bars as indicated). Microglia/macrophages are more numerous and appear larger. b Quantification of IBA1+ microglia/macrophages per ROI (n = 4–5 pups per condition). *Significant difference P = 0.011 versus control, two-tailed T test. c Shown are sections of control and IUGR rat brains at P14 labeled with CD68 (green) and DAPI (blue) and photographed at × 10 at the genu of the corpus callosum. Activated microglia/macrophages were significantly increased in this location. d Quantification of CD68+ microglia/macrophages per ROI (n = 3 pups per condition). *Significant difference P ≤ 0.001 versus control, two-tailed T test