Fig. 2

AG490 effectively upregulates MeCP2 in the cultured cortical neurons. a Representative fluorescent immunostaining images of cortical neurons after treating with AG490 or DMSO vehicle twice for 3 days each. The top row shows labeling of nuclei, and the middle row shows GFP labeling (of MeCP2-GFP). The bottom row shows the zoom-in of nuclear and MeCP2-GFP labeling from the boxed area depicted in the middle row. Arrows indicate GFP-positive cells. b Quantitative analysis of the number of GFP-positive cells (**p ≤ 0.01) (n = 4 per dose, the average of three biological replicates are shown). c GFP western blot analysis (left) and WB quantification (right) show MeCP2 upregulation in AG490- and Jaki-treated cells, *statistical significance, p ≤ 0.05 (n = 3 per compounds, the average of two biological replicates are shown)