Fig. 1
From: Long-term spatial tracking of cells affected by environmental insults
The reporter construct for long-term detection of cell lineages that experienced HSF1 activation. a The design of RFP reporter system, in which the HSE and FLPo-FRT recombination system are combined for long-term labeling. Upon exposure to stress, HSE drives the expression of FLPo, which then induces RFP expression via FRT recombination. b The HSE-FLPo fragment (2301 bp) was verified by AhdI digestion that generated the expected 1216 and 1085 bp products