Fig. 5

Contactin-3 is developmentally regulated in the human brain. A – In-situ hybridization for CNTN3 in autopsy-derived fetal and postnatal control cerebral cortex (age range: gestational week 14–67 years); B – western blot for contactin-3 in the control cortex (age range: gestational week (GW) 22–44 years); C – optical density analysis of contactin-3 expression across different ages; D – schematic representation of the SH-SY5Y cell differentiation; E – RT-qPCR showed increased expression of CNTN3 expression in differentiated SH-SY5Y cells (fold change = 6.2, p < 0.01, n = 6); GW – weeks of gestation, W – weeks, D – days, M – months, Y – years of age; RA – retinoic acid; scale bar 20 μm; **p < 0.01, Mann-Whitney U test