Fig. 5

RCAN1 knockout and overexpression elicit divergent alterations in the circadian rhythmicity of wheel running in young mice. A Plot of average daily wheel revolutions collapsed into hourly bins (floating points depicting mean ± S.E.M) with superimposed single-harmonic regression curve fits (mean ± 95% CI bands) for free-running young mice. B Mean daily MESOR estimates for wheel running rhythms of free-running young mice. Rcan1 KO mice exhibit increased daily MESOR estimates compared with Rcan1 WT and RCAN1 TG mice, while RCAN1 TG mice display decreased daily MESOR estimates relative to NTG mice. C Mean daily amplitude estimates for wheel running rhythms of free-running young mice. Rcan1 KO mice exhibit increased daily amplitude estimates compared with Rcan1 WT and RCAN1 TG mice, while RCAN1 TG mice display decreased daily amplitude estimates compared with NTG mice. D Mean daily acrophase estimates for wheel running rhythms of free-running young mice. There were no group differences in acrophase estimates. N = 17 Rcan1 WT, 21 Rcan1 KO, 17 NTG, 18 RCAN1 TG mice. All data are mean ± S.E.M. *p < 0.05; **p < 0.01; ****p < 0.0001