Fig. 1

MPS IIIB postnatal pups exhibit reduced body temperature and USVs with altered spectrotemporal features. A Timeline of developmental assessments. B Representative spectrogram segment from a Control mouse and a MPS IIIB mouse. C-D Number of ultrasonic vocalizations (USVs) during maternal isolation-induced USV assay across ages (C) and collapsed for age (D). E Body weight across postnatal assessment ages. F Latency to exhibit righting reflex at P14. G Body temperatures recorded immediately prior to USV recordings at P6-10. H Covariate-adjusted means of number of USVs accounting for differences in body temperature. I Number of phrases of USV calls. J Average USV duration. K Average pause duration between USVs within a phrase. L Sound pressure level or volume of USV calls. M Mean USV frequency. N Frequency range of USV calls. O Percent of calls that included a frequency jump greater than 10 kHz. C, E-J, M, and N data are presented as means ± SEM. D, K, L, and O data are presented as boxplots with thick horizontal lines respective group medians, boxes 25th – 75th percentiles, and whiskers 1.5 × IQR. Individual data points are presented as open circles